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KMID : 0379120140420010050
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Korean Journal of Mycology
2014 Volume.42 No. 1 p.50 ~ p.56
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Screening and Characterization of LTR Retrotransposons in the genomic DNA of Pleurotus eryngii
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Kim Sin-Il
Le Quy-Vang
Kim Sun-Mi
No Hyun-Soo
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Abstract
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Transposable elements (TEs) are mobile DNA elements that often cause mutations in genes and alterations in the chromosome structure. In order to identify and characterize transposable elements (TEs) in , a TE-enriched library was constructed using two sets of TE-specific degenerated primers, which target conserved sequences of RT and RVE domains in fungal LTR retrotransposons. A total of 256 clones were randomly chosen from the library and their insert sequences were determined. Comparative investigation of the insert sequences with those in repeat element database, Repbase, revealed that 71 of them were found to be TE-related fragments with significant similarity to LTR retrotransposons from other species. Among the TE sequences, the 70 TEs were Gypsy-type LTR retrotransposons, including 20 of MarY1 from , 26 of Gypsy-8_SLL from , and 16 of RMER17D_MM from mouse, whereas a single sequence, Copia-48-PTR, was found as only Copia-type LTR retrotransposon. Southern blot analysis of the III-digested genomic DNA showed that the retrotransposon sequences similar to MarY1 and Gypsy-8_SLL were contained as high as 14 and 18 copies per genome, respectively, whereas other retrotransposons were remained low. Moreover, both of the two Gypsy retrotransposons were expressed in full length mRNA as shown by Northern blot analysis, suggesting that they were functionally active retrotransposons.
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KEYWORD
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Degeneration, Pleurotus, Retrotransposon, Transposable element
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